The RFamide receptor DMSR-1 regulates stress-induced sleep in C. elegans
Abstract: In response to environments that cause cellular stress, animals engage in sleep behavior that facilitates recovery from the stress. In Caenorhabditis elegans, stress-induced sleep (SIS) is regulated by cytokine activation of the ALA neuron, which releases FLP-13 neuropeptides characterized by an amidated arginine-phenylalanine (RFamide) C-terminus motif. By performing an unbiased genetic screen for mutants that impair the somnogenic effects of FLP-13 neuropeptides, we identified the gene dmsr-1, which encodes a G-protein coupled receptor similar to an insect RFamide receptor. DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SIS in vivo, is expressed non-synaptically in several wake-promoting neurons, and likely couples to a Gi/o heterotrimeric G-protein. Our data expand our understanding of how a single neuroendocrine cell coordinates an organism-wide behavioral response, and suggest that similar signaling principles may function in other organisms to regulate sleep during sickness.
Iannacone, Michael J., Isabel Beets, Lindsey E. Lopes, Matthew A. Churgin, Christopher Fang-Yen, Matthew D. Nelson, Liliane Schoofs, and David M. Raizen."The RFamide receptor DMSR-1 regulates stress-induced sleep in C. elegans." eLife 6 (2017): e19837.
This document is currently not available here.